coli glycerol stocks at minus 80 or minus 20

[tarun gupta]

Hi,

Well, We use to keep the glycerol stocks at -70. But if we consider the situation when we keep the petriplates at -20, there is no further growth but we can easily revive the organisms even after a long time. So I guess, they should survive. You might wonder that in case of plates, organisms are stored over some sort of nutritional media, but dear, at -20, there is no further growth which means that they don't require "food" anymore. So this backens my point. They should survive.

Many Regards
Tarun Gupta
B.Sc (Hons) Bioinformatics
GGDSD College of post Graduation
(Panjab University)
Chandigarh-India.

http://hotbacteria.tripod.com

>Date: Wed, 12 Jul 2006 15:48:03 +0200
> From: "AS" <noreply at sefda.sed>
>Subject: coli glycerol stocks at minus 80 or minus 20
>To: methods at net.bio.net
>Message-ID: <e92uik$nj1$1 at news.net.uni-c.dk>
>
>Hi NG.
>I have all my coli glycerol stocks in 30% glycerol at minus 80! Would they
>survive if I transfer them to minus 20?
>Thanks



Tarun Gupta 
Summer Trainee
Regional Research laboratory (CSIR)
Jammu

B.Sc.(hons)Bioinformatics 
GGDSD College of Post Graduation 
(Panjab University) 
Sector-32/C 
Chandigarh-160031 
Mob: +91-9888237906

http://hotbacteria.tripod.com
http://360.yahoo.com/hotbacteria