coli glycerol stocks at minus 80 or minus 20
hotbacteria at rediffmail.com
Thu Jul 13 01:33:14 EST 2006
Well, We use to keep the glycerol stocks at -70. But if we consider the situation when we keep the petriplates at -20, there is no further growth but we can easily revive the organisms even after a long time. So I guess, they should survive. You might wonder that in case of plates, organisms are stored over some sort of nutritional media, but dear, at -20, there is no further growth which means that they don't require "food" anymore. So this backens my point. They should survive.
B.Sc (Hons) Bioinformatics
GGDSD College of post Graduation
>Date: Wed, 12 Jul 2006 15:48:03 +0200
> From: "AS" <noreply at sefda.sed>
>Subject: coli glycerol stocks at minus 80 or minus 20
>To: methods at net.bio.net
>Message-ID: <e92uik$nj1$1 at news.net.uni-c.dk>
>I have all my coli glycerol stocks in 30% glycerol at minus 80! Would they
>survive if I transfer them to minus 20?
Regional Research laboratory (CSIR)
GGDSD College of Post Graduation
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