Help: low transfection efficiency but control works great

Yulia Shifrin yulia24 at gmail.com
Thu Jun 1 12:46:20 EST 2006


 Hello everybody!
I'm having problems with transfecting melanoma cells with vector containing
dsRed fused to my protein of interest. I'm using Fugene 6 and get 60-70%
efficiency with vector-only control, but only 1-5% efficiency with my
construct. The protein that I'm working on is pretty large (300 kDa). I'm
getting really frustrated. Has anybody encountered similar problems? What
can i do to improve the efficiency
Thanks in advance
 Yulia
<methods at net.bio.net>


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