Prolems detecting protein with antibodies

Allison allison at nospam.com
Fri Jun 2 09:13:59 EST 2006


[snip]
> 
> Dear Allison,
> 
> Thanks for your suggestions. We are not able to see the protein on a 
> commassie stained gel even from it's intrinsic promoter, so the 
> expression is obviously very low, probably for biological reasons (the 
> protein is perhaps toxic to the host cell when in high concentrations). 

So how do check that the protein is present if you can't see it on a 
gel?  You could try doing the same test for your new construct.


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