Prolems detecting protein with antibodies
allison at nospam.com
Fri Jun 2 09:13:59 EST 2006
> Dear Allison,
> Thanks for your suggestions. We are not able to see the protein on a
> commassie stained gel even from it's intrinsic promoter, so the
> expression is obviously very low, probably for biological reasons (the
> protein is perhaps toxic to the host cell when in high concentrations).
So how do check that the protein is present if you can't see it on a
gel? You could try doing the same test for your new construct.
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