Impossible PCR product cloning

Jayakumar, R R.Jayakumar at roswellpark.org
Sun Jun 4 09:11:24 EST 2006


It would be helpful, if you can let us know the protocol you follow with enzyme conc., make,  volume, amount of vector and insert, temperatures and time whether CIAP was used or not etc.  Detailed..
jai


                       
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