proper coprecipitant for primer extension
tandna at yahoo.com
Sun Jun 4 15:03:35 EST 2006
im doing primer extension of some tRNAs. Facing one problem with my in vitro-transcribed control, i can get the full lenght signal but with a very strong background.
I used total yeast RNA as coprecipitant in that reaction, not sure if this background is coming from inespecific binding of my primer to the yeast RNA.
Anybody as experience on the use of primer extension for tRNAs?
Thanks in advance,
Tania Bibiana Lombo Rodriguez
Graduated Student-RNA Decay and Processing
Max F. Perutz Laboratories
Departments at the Vienna Biocenter
Institute for Microbiology and Immunobiology
email:Tania.Lombo at univie.ac.at
Dr.Bohr.G. 9, Campus Vienna Biocenter 4
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