10% sarkosyl? Can't get it in!
R.Jayakumar at roswellpark.org
Wed Jun 7 13:33:37 EST 2006
Much Better, if you can autoclave it. That will dissolve it very well.
I used to do that a long time when I used Sarkosyl in my DNA extraction
buffer. Sarkosyl does not decompose upon autoclaving.
From: methods-bounces at oat.bio.indiana.edu
[mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of DK
Sent: Tuesday, June 06, 2006 11:08 PM
To: methods at magpie.bio.indiana.edu
Subject: Re: 10% sarkosyl? Can't get it in!
In article <e643oj$leh$1 at gemini.csx.cam.ac.uk>, C Coward
<cc122 at mole.bio.cam.ac.uk> wrote:
>I'm trying to make some 10% sarkosyl (N-lauryl sarcosine). It should be
>possible, should it not at 10% (w/v) in water. It doesn't appear to be
>going in, despite stirring/heating. Admittedly our container of
>is rather old, and it had set together somewhat. Should I buy some new,
>are there any tricks? I may decide to make it up at 5% if I can't get
>in, but 10% would be more convenient.
I remember making 10% stock some time ago. It took something like
overnight stirring ar RT. Make sure yours is sodium salt. A different
might have different solubility.
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