discriminating SOD1 and SOD2 activities

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Thu Jun 8 07:13:53 EST 2006


cloningman wrote:


> Then upper phase (acqueous phase) is recovered and immediately assayed for
> SOD enzymatic activity (Sun & Zigman, 1978) and for protein content
> (Bradford method).

IIRC ethanol is incompatible with Bradford protein determination. You'd
have to either precipitate the protein in your supernatant or choose
another method (e.g. bicinchonic acid).


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