Buffer preperation

Han nobody at nospam.not
Sun Jun 11 14:59:30 EST 2006


daoud abusafieh <daoud_il at yahoo.com> wrote in
news:mailman.78.1150053713.17804.methods at net.bio.net: 

> Kindly I want to ask how to prepare 1 liter buffer solution of 66mM
> KH2PO4/K2HPO4 pH 7. 
>    
>   Best thanks
>    
>   Daoud
> 
> 
> Daoud AbuSafieh
> Faculty of Agriculture 
> University of Thessaly 
> Volos 
> Greece
> 
> 
Make 1 L each KH2PO4 and K2HPO4 
Take 100 ml KH2PO4, then add K2HPO4 solution while stirring to bring pH to 
7.4.  Measure how much you used.  Then mix appropriate amounts to end up 
with 1 L

Or you could look it up using google, for example:
<http://www.fisher.co.uk/phpfiles/techzone/question.php?questionid=82>
-- 
Best regards
Han 
email address is invalid


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