RT-PCR on 10,000 cells

R Lakshman Chelvarajan Lakshman.C at uky.edu
Tue Jun 13 09:56:28 EST 2006

I can only get a few purified cells each time. Is it possible to obtain meaningful RT-PCR data from only 10,000 primary murine cells? If so, can someone recommend a paricular kit or technique?


R. Lakshman Chelvarajan <RCHEL1 at uky.edu>
PH +1 859 257 1412 ext 268; Fax +1 859 323 2866
Center on Aging, University of Kentucky, Lexington KY 40536-0230, U.S.A., Earth

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