Does the postPCR 4-degree hold damage the thermal cycler ??

arnaud.garcon at arnaud.garcon at
Fri Jun 16 03:07:16 EST 2006

> Completely unecessary anyway; samples have already been 
> cycled to 94 degrees 30 or so times, sitting at rt overnight 
> doesn't harm your samples one bit.
A good point, but what if you're using a proof reading polymerase with
exonuclease activity. Don't you risk your product being chewed up?


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