Does the postPCR 4-degree hold damage the thermal cycler ??
lhom at OCF.Berkeley.EDU
Thu Jun 15 20:30:54 EST 2006
I have no evidence that an overnight 4deg hold causes excessive wear on a
thermocycler, but having touched the hot side of a peltier module cooling
to 4deg, it wouldn't at all surprise me if it did present a problem.
FWIW, I have done many overnight 10deg holds without ill effect on the
DNA. I don't know if it's any better for the instrument, but it makes me
Lou Hom >K'93
lhom at ocf.berkeley.edu
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