Does the postPCR 4-degree hold damage the thermal cycler ??

Louis Hom lhom at OCF.Berkeley.EDU
Thu Jun 15 20:30:54 EST 2006

I have no evidence that an overnight 4deg hold causes excessive wear on a 
thermocycler, but having touched the hot side of a peltier module cooling 
to 4deg, it wouldn't at all surprise me if it did present a problem.

FWIW, I have done many overnight 10deg holds without ill effect on the 
DNA.  I don't know if it's any better for the instrument, but it makes me 
feel better.
Lou Hom >K'93			     
lhom at 	    

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