Does the postPCR 4-degree hold damage the thermal cycler ??

chovek69 ivanoov at gmail.com
Fri Jun 16 02:22:58 EST 2006


Thanks all,

I have searched the manuals of all of our 5 different PCR machines and
there were no even a slight note on the possible damage of 4 deg.
holds.

Rory O'Brien wrote:
> I think its more to do with the condensation which forms underneath the
> block; can't be good for it...
>
> Completely unecessary anyway; samples have already been cycled to 94 degrees
> 30 or so times, sitting at rt overnight doesn't harm your samples one bit.
>
> - Rory
>
> > -----Original Message-----
> > From: methods-bounces at oat.bio.indiana.edu
> > [mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of Louis Hom
> > Sent: 16 June 2006 13:31
> > To: methods at magpie.bio.indiana.edu
> > Subject: Re: Does the postPCR 4-degree hold damage the
> > thermal cycler ??
> >
> > I have no evidence that an overnight 4deg hold causes
> > excessive wear on a thermocycler, but having touched the hot
> > side of a peltier module cooling to 4deg, it wouldn't at all
> > surprise me if it did present a problem.
> >
> > FWIW, I have done many overnight 10deg holds without ill
> > effect on the DNA.  I don't know if it's any better for the
> > instrument, but it makes me feel better.



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