denaturing buffer for HPLC

Josmar josmarlangner at yahoo.de
Fri Jun 23 06:37:34 EST 2006


OK,
I tried 7M urea in PBS in place of 100 mM triethylamine. The same thing
happens. The protein that comes off the superdex 200 column at~13 ml
under native conditions (PBS only) elutes at the column's void volume
of ~8ml with 7M urea as running buffer. I have no idea what to think
about this.
Josmar



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