How to dispose beta-Mercaptoethanol out of solutions

Sebastian Gruenberg sebastian.gruenberg at biologie.uni-regensburg.de
Tue Jun 27 06:46:17 EST 2006


Hi members,

I have an urgent question for the forum.

I'm doing KMnO4-footprinting experiments and unfortunately, BME 
disturbes the KMnO4 reaction (it is also used to quench the reaction 
later). Since there is BME in the buffers of the proteins used in my 
reactions, I have to get rid of almost all the BME in the protein 
solutions.

Does anybody now how to do this without damaging the sensitive 
proteins?

Thank you very much.

Sebastian

--

Dipl.-Biol. Sebastian Grünberg
Archaeenzentrum &
Lehrstuhl für Mikrobiologie
Universität Regensburg
Universitätsstrasse 31
93053 Regensburg; Germany
Tel.: ++49-941-943 3146
Fax.: ++49-941-943 2403



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