How to dispose beta-Mercaptoethanol out of solutions

Austin P. So (Hae-Jin) nobody at nowhere.com
Tue Jun 27 12:34:41 EST 2006


Sebastian Gruenberg wrote:
> I'm doing KMnO4-footprinting experiments and unfortunately, BME 
> disturbes the KMnO4 reaction (it is also used to quench the reaction 
> later). Since there is BME in the buffers of the proteins used in my 
> reactions, I have to get rid of almost all the BME in the protein 
> solutions.

You could use a microdialyser with a molecular weight cutoff of about 
1/5 the size of your protein. Alternatively, you can use a 
microfiltration unit to buffer exchange (YM-3 up to YM-100 from Millipore)

Austin


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