Help!!!Restriction enzyme digestion troubles.
hzhen at freeuk.com
Tue Jun 27 21:45:50 EST 2006
On Mon, 26 Jun 2006 08:03:45 +0100 (BST), shyam nandi
<biotech_iicb at yahoo.co.in> wrote:
>i am doing double digest restriction digestion of baculogold vector with
> RE pstI & kpnI
> the both sites are contigously present one after another in the vector
> .....CTGCAGGGTACC...... (vector)
> pstI I kpnI
> Is my digestion should complete successfully.
Probably not. Digest with PstI first, then KpnI.
Check NEB catalog for information on how many bases are required for
> Please reply
> i am not getting any colony after ligation & transformation
> While getting colony in non contiguously present RE site,using same vector
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