blackhole at abuse.plus.com
Wed Jun 28 04:40:46 EST 2006
Historians believe that in newspost
<mailman.20.1151434124.20007.methods at net.bio.net> on Tue, 27 Jun 2006,
Aníbal Acuña <aacuna152 at gmail.com> penned the following literary
>But then, with higher gDNA amounts (over 1E6
>copies), bands of higher molecular weight appear (the product is there
>How can I explain the appearance of those unexpected bands?.
Think about it.
10E6 copies of genomic DNA is a lot!
If it was human DNA it would be 3ug of genomic DNA!
So if you stick loads of genomic DNA in a PCR then run the finished
amplification out on the gel, don't be surprised to see the starting
genomic DNA in an aliquot of the final PCR run out on a gel.
> Why is it that
>if I increase the amount of gDNA, those bands disappear?
Disappear or appear?
Appear because of above.
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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