Restriction sites.

Ezhil Subbian eksubbs at
Wed Jun 28 16:58:09 EST 2006


I designed primers to add new restriction sites but forgot to add any "clamp" sequences at either end. I have a KpnI site at one end and a BamHI site at the other.  I realise my mistake. I'm curious to see if anyone has had any succes with digesting inserts with the restirction site at the very end.  Or if its a lost case and I should just start over with fresh primers.  


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