denaturing buffer for HPLC

Josmar josmarlangner at yahoo.de
Fri Jun 30 04:50:37 EST 2006


Hi Roland,
I am not sure if I understand what you are saying. What comes off the
column at the void volume must be really huge, i.e. aggregated. Can you
explain what you mean by swept volume?
Thanks
Josmar

> The protein may be eluting in the void volume because the swept volume
> of the unfolded protein is much larger than the folded protein.  How do
> you know it is aggregating?
>
> Analytical ultracentrifugation may be a better way to look at monomer
> dimer equilibria and similar questions.  
> 
> Roland



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