denaturing buffer for HPLC

Josmar josmarlangner at
Fri Jun 30 04:50:37 EST 2006

Hi Roland,
I am not sure if I understand what you are saying. What comes off the
column at the void volume must be really huge, i.e. aggregated. Can you
explain what you mean by swept volume?

> The protein may be eluting in the void volume because the swept volume
> of the unfolded protein is much larger than the folded protein.  How do
> you know it is aggregating?
> Analytical ultracentrifugation may be a better way to look at monomer
> dimer equilibria and similar questions.  
> Roland

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