restriction digst not working

Pow Joshi pow.joshi at gmail.com
Thu Jun 29 23:34:29 EST 2006


>I am digesting the plasmid DNA which has got subclobned DNA,but when
>iamdigesting with the same enzymes used for subcloning,not atatll
>digesting,and it is not having reverse clonable probabilities.And two month
>old plamid getting digestion,but trhe same is not dgesting now,is that
probl
>of  any components,becos recently we changed Phenol,and water,the waster
>from MQ which shown" change catridge">is that problem of water ,please any
>one suggest  to rectify.

Hi,
would you please specify the details, eg: what you see in the gel, what size
fragment you're supposed to have; are you trying to digest a new plasmid
prep etc...... we could troubleshoot better.

pow

On 6/29/06, suresh kumar <ramsuresh74 at hotmail.com> wrote:
>
>
> I am digesting the plasmid DNA which has got subclobned DNA,but when
> iamdigesting with the same enzymes used for subcloning,not atatll
> digesting,and it is not having reverse clonable probabilities.And two
> month
> old plamid getting digestion,but trhe same is not dgesting now,is that
> probl
> of  any components,becos recently we changed Phenol,and water,the waster
> from MQ which shown" change catridge">is that problem of water ,please any
> one suggest  to rectify.
> BRS
> <html><DIV>
> <P><FONT face="Lucida Handwriting, Cursive" color=#ff3333 size=4><FONT
> face="Times New Roman, Times, Serif">R.Suresh Kumar </FONT><IMG height=19
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> width=19><BR></FONT>Molecular genetics lab, <BR>Institute of cytology and
> preventive oncology(ICMR) <BR>I-7 sector 39,Near Govt PG
> college
> NOIDA (U.P)
> 201301
> INDIA</P>
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>
>
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