PCR Self Adjusting Magnesium concentration

Tim Fitzwater tfitzwater at somalogic.com
Fri Jun 30 17:35:38 EST 2006


A quick check of the MSDS for this product reveals that the secret chelator is L-proline at somewhere between 1 and 5% (w/v) for 25 mM magnesium acetate at the 10x buffer concentration.  I have not tried it yet.

Tim Fitzwater


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