The making of a DNA construct with deletions?

as nospam at nospam.com
Thu Mar 2 04:29:31 EST 2006


Dear NG.
I have a 1500bp DNA construct where I want to delete 5-6 smaller regions 
dispersed throughout the sequence. What is the easiest way to do this? Is it 
by amplifying the different cassettes of DNA with flanking restrictions 
sites and then joining them, or is there another way to get around? Any help 
would be appreciated. Thanks.





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A. Salanti
University of Copenhagen
Blegdamsvej 3C,
2200 Copenhagen
Denmark (Sorry)
 




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