The making of a DNA construct with deletions?
king-ferdi at gmx.net
Thu Mar 2 05:51:44 EST 2006
if you have single cutter flanking the sites of interest, just digest
with the appropiate restriction endonucleases. Then create blund ends
with T4 Polymerase and rejoin them with T4 ligase.
In article <du6d9t$sdo$1 at news.net.uni-c.dk>, nospam at nospam.com says...
> Dear NG.
> I have a 1500bp DNA construct where I want to delete 5-6 smaller regions
> dispersed throughout the sequence. What is the easiest way to do this? Is it
> by amplifying the different cassettes of DNA with flanking restrictions
> sites and then joining them, or is there another way to get around? Any help
> would be appreciated. Thanks.
> A. Salanti
> University of Copenhagen
> Blegdamsvej 3C,
> 2200 Copenhagen
> Denmark (Sorry)
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