The making of a DNA construct with deletions?
nospam at nospam.com
Fri Mar 3 05:29:08 EST 2006
"Stacy Ferguson" <stacyef at stacyef.net> wrote in message
news:stacyef-22DCE8.22394202032006 at news.newsguy.com...
> In article <du6d9t$sdo$1 at news.net.uni-c.dk>, "as" <nospam at nospam.com>
>> Dear NG.
>> I have a 1500bp DNA construct where I want to delete 5-6 smaller regions
>> dispersed throughout the sequence. What is the easiest way to do this? Is
>> by amplifying the different cassettes of DNA with flanking restrictions
>> sites and then joining them, or is there another way to get around? Any
>> would be appreciated. Thanks.
> How big are the regions you want to delete?
> Are you making all five deletions in one sequence or are you making a
> set of five mutants?
I want to make all mutations in one construct. The deletion are between 4
and 15 aa.
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