Question about Promoter of pBR322 ROP

AdV wisy98 at yahoo.com.cn
Sat Mar 4 03:21:55 EST 2006


>From Current Protocols and Maniatis, I have found that the rop is
responsible for the low copies numbers of plasmid. Now I would like to
know if the promoter of Rop and Ter is the same as P2 ? I find that
only 3 proter were reported. The direction of Ter , Rop and P2 is
same.If I delete the P2 from pBR322, the copies numbers of the plasmid
in the cells increase or decrease?



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