Right solvent to dissolve ampicillin ?

Siddhartha Parida sidharth4 at gmail.com
Sat Mar 4 07:28:52 EST 2006


My ampicillin sod.salt has expired (exp date june 2004 ) will it be
viable still ? therefore  i am trying with ampicillin capsules found
in medicine stores , but after getting the calculation right it is not
dissolving in water nor alcohol , can anyone suggest me the right
solvent

I will be using this stock solution of ampicillin in LBA medium for a
alpha complementation protocol

On 2/28/06, methods-request at oat.bio.indiana.edu
<methods-request at oat.bio.indiana.edu> wrote:
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> Today's Topics:
>
>   1. Re: protein heterodimer seperated during purification with
>      superdex200 (DK)
>   2. Primers, DDWater, TE Buffer (Alvin Alejandrino)
>   3. Re: TUNEL (DK)
>   4. Mass Ladder (ali)
>   5. Re: Primers, DDWater, TE Buffer (Christian Praetorius)
>   6. looking for non radioactive kinase assays (Carlo Zambonelli)
>   7. the condition of electroporating MDBK (Zhou Xiaoyan)
>   8. DNA digest in gel (cheetah.zao at gmail.com)
>   9. Re: Primers, DDWater, TE Buffer (GysdeJongh)
>  10. Re: Primers, DDWater, TE Buffer (Rajesh Kumar Singh)
>  11. Re: Primers, DDWater, TE Buffer (Christian Praetorius)
>  12. Re: Primers, DDWater, TE Buffer (Christian Praetorius)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 27 Feb 2006 14:21:51 GMT
> From: dk at no.email.thankstospam.net (DK)
> Subject: Re: protein heterodimer seperated during purification with
>        superdex200
> To: methods at net.bio.net
> Message-ID: <dtv1tv$kto$1 at news.doit.wisc.edu>
>
> In article <1141031176.320387.225840 at t39g2000cwt.googlegroups.com>, "cheetah.zao at gmail.com" <cheetah.zao at gmail.com> wrote:
> >hi,everyone
> >
> >I have been working on purifying a protein heterodimer using affinity
> >column(one of target protein has a GST tag) and superdex200.
> >
> >the first part went well, I got two bands in SDS gel, however, I met
> >something weird when using superdex200 to purify the eluents of the
> >affinity column: there were two main peaks,with one of my target
> >protein seperately.
> >
> >Is it quit unusual for superdex200 to drag protein complex apart,
> >
> >has anyone met this too?
>
> Alll the time. It's not that gel-filtration matrix "drags protein complex
> apart" but the fact that your complex is of relatively low affinity.
> For those, hal-life time of the complex is small (say, minutes instead of
> hours or days), thus if the the two proteins are considerably different
> in size, they eventually separate.
>
> The situation is compeltely different in affinity chromatography,
> where one of the components is always at high concentration
> (> or >>Kd, ~ 0.1 mM is typical).
>
> DK
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 27 Feb 2006 10:18:44 -0800
> From: "Alvin Alejandrino" <aalejandrino at nhm.org>
> Subject: Primers, DDWater, TE Buffer
> To: <methods at magpie.bio.indiana.edu>
> Message-ID: <EE6DFADE289313489E832D79B2C5E45D0242CD19 at hermes.nhm.lac>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> Hey everyone,
>
> Normally, I use TE Buffer to dilute and resuspend primers from the manufacturer.  Some people have told me that Double Distilled Water is an okay and easy way to dilute the primers.  Any thoughts?  It seems way too easy.  What problems will I encounter?
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 27 Feb 2006 14:29:09 GMT
> From: dk at no.email.thankstospam.net (DK)
> Subject: Re: TUNEL
> To: methods at net.bio.net
> Message-ID: <dtv2bm$kto$2 at news.doit.wisc.edu>
>
> In article <EpBMf.17322$yK1.5244 at news-server.bigpond.net.au>, "newsnet customer" <customer at newsnet.com> wrote:
> >This is a multi-part message in MIME format.
> >
> >------=_NextPart_000_0011_01C63BE9.1744EE50
> >Content-Type: text/plain;
> >        charset="iso-8859-1"
> >Content-Transfer-Encoding: quoted-printable
>
> Please only post plaintext messages on Usenet. Thank you.
>
> >Is anyone familiar with TUNEL assay.
> >All i know is that it detects fragmented DNA.
> >Anyone know how it works?
>
> It works by incorporating labeled (usually fluorescent)
> nucleotide (usuallly dUTP) to the termini of DNA by
> a specific enzyme (TdT terminal transferase).
> The more free ends (as in apoprosis where DNA is
> fragmented), the higher incorporation, the higher
> signal.
>
> DK
>
>
> ------------------------------
>
> Message: 4
> Date: 27 Feb 2006 13:09:03 -0800
> From: "ali" <atahery at gmail.com>
> Subject: Mass Ladder
> To: methods at net.bio.net
> Message-ID: <1141074543.829968.265400 at p10g2000cwp.googlegroups.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Do we have to buy the mass ladder all the time or it is possible to
> make our own. Except spect and Gel,  is there any reliable method is
> quantifying DNA?
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 27 Feb 2006 21:37:16 +0000
> From: Christian Praetorius <prae at gmx.net>
> Subject: Re: Primers, DDWater, TE Buffer
> To: methods at net.bio.net
> Message-ID: <46h9obFbaabjU1 at individual.net>
> Content-Type: text/plain; charset=us-ascii
>
> "Alvin Alejandrino" <aalejandrino at nhm.org> wrote:
>
> >Normally, I use TE Buffer to dilute and resuspend primers from the manufacturer.  Some people have told me that Double Distilled Water is an okay and easy way to dilute the primers.  Any thoughts?  It seems way too easy.  What problems will I encounter?
>
> I think, it doesn't matter. TE may be better because it is buffered
> and my complex ions, which are necessary for nucleases. I always
> dissolve and dilute my primers in double distilled water and this
> worked always.
>
> Christian
>
> --
>        [X] <-- nail here for new monitor
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 27 Feb 2006 19:06:53 -0500
> From: "Carlo Zambonelli" <zambonel at gmail.com>
> Subject: looking for non radioactive kinase assays
> To: methods at magpie.bio.indiana.edu
> Message-ID:
>        <ad3c93330602271606v59d9f338kca6bf1071bb0c1a1 at mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear all,
> I am looking for a non radioactive universal kinase assay kit. I found many
> companies selling kits apparently suitable for this application: I would
> like to receive recommendations/suggestions from first hand users of any
> such kit. From your experience, what is the sensitivity compared to
> radioactive methods?
> Thanks,
> Carlo
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 28 Feb 2006 10:37:07 +0800
> From: "Zhou Xiaoyan" <zxy780405 at gmail.com>
> Subject: the condition of electroporating MDBK
> To: methods at magpie.bio.indiana.edu
> Message-ID: <30463dd60602271837j4e26f01k at mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi
>    I'm from China, I have found the electroporation of MDBK on internet.
> Can you tell me the condition of electroporation? Thank you!
>
> your oversea friend
>
> Xiaoyan Zhou
>
>
> ------------------------------
>
> Message: 8
> Date: 28 Feb 2006 00:46:52 -0800
> From: "cheetah.zao at gmail.com" <cheetah.zao at gmail.com>
> Subject: DNA digest in gel
> To: methods at net.bio.net
> Message-ID: <1141116412.101620.147830 at u72g2000cwu.googlegroups.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> hi  everyone, I'm new to Molecular biology.
> In order to reduce unnecessary yield loss, I would like to  digest DNA
> in normal Agarose gel 1%, should I make some adjustment of the reagents
> compared to auqatic digestion, or anyone who is familiar with it has
> other advices?
>
>
> zhao
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 28 Feb 2006 16:06:08 +0100
> From: "GysdeJongh" <jongh711 at planet.nl>
> Subject: Re: Primers, DDWater, TE Buffer
> To: methods at net.bio.net
> Message-ID: <440466dd$0$2015$ba620dc5 at text.nova.planet.nl>
>
> "Christian Praetorius" <prae at gmx.net> wrote in message
> news:46j1piFbdg6eU1 at individual.net...
> > Christian Praetorius <prae at gmx.net> wrote:
> >
> >>I always
> >>dissolve and dilute my primers in double distilled water and this
> >>worked always.
> >
> > One more thing: I always dilute the primer stocks to 100pmol and make
> > a 10pmol dilution for use in PCR.
>
> Hi,
> I  _think_  you mean 100 pmol / microliter , which is 100 micromolar or 100
> micromol / liter
> To avoid the greek alphabet  :)
> hth
> Gys
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 28 Feb 2006 10:05:12 +0100
> From: Rajesh Kumar Singh <rsingh at EMBL-Hamburg.DE>
> Subject: Re: Primers, DDWater, TE Buffer
> To: methods at magpie.bio.indiana.edu
> Message-ID:
>        <Pine.SGI.4.44.0602281002300.1986127-100000 at butthead.embl-hamburg.de>
> Content-Type: TEXT/PLAIN; charset=US-ASCII
>
> usually its not a problem in distilled water. but its good to
> keep track of pH of water being used.
>
> Rajesh Kumar Singh
> European Molecular Biology Laboratory
> C/o DESY, Notkestrasse 85, Geb. 25A
> 22603 Hamburg, Germany
> E-mail: rsingh at embl-hamburg.de
>
> On Mon, 27 Feb 2006, Christian Praetorius wrote:
>
> > "Alvin Alejandrino" <aalejandrino at nhm.org> wrote:
> >
> > >Normally, I use TE Buffer to dilute and resuspend primers from the manufacturer.  Some people have told me that Double Distilled Water is an okay and easy way to dilute the primers.  Any thoughts?  It seems way too easy.  What problems will I encounter?
> >
> > I think, it doesn't matter. TE may be better because it is buffered
> > and my complex ions, which are necessary for nucleases. I always
> > dissolve and dilute my primers in double distilled water and this
> > worked always.
> >
> > Christian
> >
> > --
> >         [X] <-- nail here for new monitor
> > _______________________________________________
> > Methods mailing list
> > Methods at net.bio.net
> > http://www.bio.net/biomail/listinfo/methods
> >
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 28 Feb 2006 13:33:42 +0000
> From: Christian Praetorius <prae at gmx.net>
> Subject: Re: Primers, DDWater, TE Buffer
> To: methods at net.bio.net
> Message-ID: <46j1piFbdg6eU1 at individual.net>
> Content-Type: text/plain; charset=us-ascii
>
> Christian Praetorius <prae at gmx.net> wrote:
>
> >I always
> >dissolve and dilute my primers in double distilled water and this
> >worked always.
>
> One more thing: I always dilute the primer stocks to 100pmol and make
> a 10pmol dilution for use in PCR.
>
> Christian
>
> --
>        [X] <-- nail here for new monitor
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 28 Feb 2006 15:28:55 +0000
> From: Christian Praetorius <prae at gmx.net>
> Subject: Re: Primers, DDWater, TE Buffer
> To: methods at net.bio.net
> Message-ID: <46j8hkFb5bb5U3 at individual.net>
> Content-Type: text/plain; charset=us-ascii
>
> "GysdeJongh" <jongh711 at planet.nl> wrote:
>
> >I  _think_  you mean 100 pmol / microliter
>
> Argh, you are right.
>
> Christian
>
> --
>        [X] <-- nail here for new monitor
>
>
> ------------------------------
>
> _______________________________________________
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>
> End of Methods Digest, Vol 9, Issue 28
> **************************************
>


--
Sidharth Parida
Senior Faculty
Center for biotechnology and Research
Neelachal Inst of Medical Sciences
O.C.H.C building , Near Ram Mandir
Bhubaneswar-3
ORISSA
09437089337(M)
sidharth4 at gmail.com



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