plasmid agarose electrophoresis

Jayakumar, R R.Jayakumar at roswellpark.org
Thu Mar 16 10:04:52 EST 2006


Try a 6% agarose gel.  A very long one preferably as big as 15 - 20 Cm
long.  Prestain the gel and run them till you can observe sufficient
separation of the bands.  That should do.   
   That should work. 

-----Original Message-----
From: methods-bounces at oat.bio.indiana.edu
[mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of vagos
Sent: Wednesday, March 15, 2006 3:25 PM
To: methods at magpie.bio.indiana.edu
Subject: plasmid agarose electrophoresis

Hi, everyone, my name is Evagelos Liapis and I am a PhD student at the
university of Leicester. I have the following question:

I would like to separate a 4900bp from a 5014bp linear plasmid. Which
agarose would you recommend for that experiment and in what percentage?
Thank you in advance,
Evagelos Liapis

_______________________________________________
Methods mailing list
Methods at net.bio.net
http://www.bio.net/biomail/listinfo/methods


This email message may contain legally privileged and/or confidential information.  If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited.  If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.



More information about the Methods mailing list