plasmid agarose electrophoresis

Jayakumar, R R.Jayakumar at
Thu Mar 16 10:04:52 EST 2006

Try a 6% agarose gel.  A very long one preferably as big as 15 - 20 Cm
long.  Prestain the gel and run them till you can observe sufficient
separation of the bands.  That should do.   
   That should work. 

-----Original Message-----
From: methods-bounces at
[mailto:methods-bounces at] On Behalf Of vagos
Sent: Wednesday, March 15, 2006 3:25 PM
To: methods at
Subject: plasmid agarose electrophoresis

Hi, everyone, my name is Evagelos Liapis and I am a PhD student at the
university of Leicester. I have the following question:

I would like to separate a 4900bp from a 5014bp linear plasmid. Which
agarose would you recommend for that experiment and in what percentage?
Thank you in advance,
Evagelos Liapis

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