protein expression in BL21

kaj.stenberg at kaj.stenberg at
Sat Mar 18 15:32:24 EST 2006

khanoon110 at wrote:
> I use pET17b to express my protein in BL21. At first everything is
> fine, I could get overproduction of the protein but after 1-2 monthes
> later the level of expression is decrease. Has anyone ever face this
> problem?

Quite normal. Always use a fresh strain from -70.


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