conversion of rate to kcat?

matsn j.nilsson at ucl.ac.uk
Tue Mar 21 06:55:16 EST 2006


Hi! Thanks for your answer.

However,
I want to express the enzymatic rate (or V) as kcat at different points
during a reactions.

For example: The concentration of the enzyme is 1.5mg/ml and the
activity is 72 nmol/min at 5 mintues into the reaction. Then what is
the kcat at that point of the reaction.

Is this possible? Or do i need a vmax value to work around?

/Regards, Joakim



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