B. megaterium transformation

Matt Hymes mhymes at oxfordbiomed.com
Tue Mar 21 09:16:32 EST 2006


Hello,

I am trying to transform Bacillus megaterium protoplasts purchased from 
a commercial source and have been unsuccessful. Does any have 
experience with this? I am wondering if the source of the plasmid DNA 
matters, i.e. can it be from a mini prep using a commercial kit such as 
Qiagen?

Cheers,
Matthew



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