ligation reaction

[as]

"Duncan Clark" <blackhole at abuse.plus.com> skrev i en meddelelse 
news:2J7HYhBqRAJEFA11 at abuse.plus.com...
> Historians believe that in newspost 
> <0XRUf.15432$dy4.7044 at news-server.bigpond.net.au> on Fri, 24 Mar 2006, 
> newsnet customer <customer at newsnet.com> penned the following literary 
> masterpiece:
>>Or simply dilute the ligation mix and transform into E.coli?
>
> 10ul ligation plus 20ul water and heat kill 65C for 20mins. Can EtOH ppt 
> or use 1ul direct.
>
> Duncan
> -- 
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
>
> Duncan Clark
> GeneSys Ltd.

Hi.
Do you heat kill you ligation mix?

A.s.