ligation reaction

as nospam at
Fri Mar 24 11:42:59 EST 2006

"Duncan Clark" <blackhole at> skrev i en meddelelse 
news:2J7HYhBqRAJEFA11 at
> Historians believe that in newspost 
> <0XRUf.15432$dy4.7044 at> on Fri, 24 Mar 2006, 
> newsnet customer <customer at> penned the following literary 
> masterpiece:
>>Or simply dilute the ligation mix and transform into E.coli?
> 10ul ligation plus 20ul water and heat kill 65C for 20mins. Can EtOH ppt 
> or use 1ul direct.
> Duncan
> -- 
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
> Duncan Clark
> GeneSys Ltd.

Do you heat kill you ligation mix?


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