qPCR NEWSLETTER - March 2006

Editor www.Gene-Quantification.info editor at gene-quantification.info
Tue Mar 28 05:45:48 EST 2006

qPCR NEWSLETTER  - March 2006

Dear researcher,
dear Gene Quantification page user,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarized on the Gene
Quantification homepage. In this Newsletter issue:  FREE download of
the qPCR 2005 Event Proceedings, Talks, Posters and very nice Photo

qPCR 2005 Symposium Proceedings  ( ISBN 3000166874 )
2nd Int. qPCR Symposium & Application Workshop in
Freising-Weihenstephan 2005
qPCR 2005 Homepage
Download Talks and Poster presentations
Photo Gallery



Please visit our independent  Academic & Industrial Information
Platform for qPCR platform

Please visit our independent  Academic & Industrial Information qPCR
Platform - The reference in qPCR.
 A lot of qPCR companies and independent institutions participate in
our qPCR information platform. On the company sub-pages new real-time
cyclers, consumables, new released kits and detection chemistries, as
well as innovative amplification technologies are presented. Links from
the sub-pages will lead you to the related product pages at the
respective homepage. You are welcome to join our Academic & Industrial
qPCR platform with your company or institution. For further info please

qPCRplatform at gene-quantification.info


Russell Higuchi
"Pioneer in real-time PCR", Associate Director of the Human Genetics
Department, Roche Molecular Systems, Almeda, USA
Real-time PCR, a personal perspective.

Mikael Kubista
Professor of Biotechnology, MultiD Analyses AB, Göteborg, Sweden
Classification of real-time PCR data.

Michael W. Pfaffl
Reader in Physiology, Center of Life Science, Weihenstephan, Technical
University of Munich, Germany
Influence of RNA matrix effect on qRT-PCR results - an overview.

Greg Shipley
Director, Quantitative Genomics Core, Laboratory, The University of
Texas Health Science Centre- Houston, USA
Design and optimization of Taqman and SYBR Green I real-time qPCR

Pamela Scott Adams
Director, Molecular Biology Core Facility, Trudeau Institute,Saranac
Lake, NY, USA
A Comparison of Real-Time RT-PCR Technique, Chemistries and
Instrumentation in Laboratories Utilizing the Same Assay.

Jo Vandesompele
Center for Medical Genetics Ghent, Ghent University Hospital, Ghent,
Normalization of gene expression: state of the art and preview on a new
strategy using expressed Alu repeats.

Jan Hellemans
Center for Medical Genetics Ghent, Ghent University Hospital, Ghent,
qBase: relative quantification software for management and automated

Bernadett Papp
Gene Expression Unit, EMBL, Heidelberg, Germany
ChIP experiments from Drosophila tissues analyzed by qRT-PCR.

Anders Stahlberg
Department of Chemistry and Biosciences, Chalmers University of
Technology, Göteborg, Sweden
Single Cell Transcriptome.

Ron Jenkins
Proficiency Program: A Summary of Participants Capabilities for
Detecting and Quantifying Transgenic Events in Corn and Soybeans.

Philipp Hübner
Kantonales Laboratorium Basel-Stadt, Abteilungsleiter Lebensmittel,
Basel, Switzerland
Uncertainties and certainties in GMO analytics using qPCR.

Isabel Taverniers
Ministry of the Flemish Community,Centre for Agricultural
ResearchCLO,Dept. for Plant Genetics and Breeding - DvP, Belgium
Cloned plasmid DNA molecules as a tool for GMO analysis.

Francisco Moreano
Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit
(LGL),Infektologie / Molekularbiologie, Germany
Application of synthetic DNA-standards for the quantitative screening
of different genetically modified rapeseed lines via real-time PCR.

Kristina Lind
Department of Chemistry and Biosciences, Chalmers University of
Technology, Göteborg, Sweden
Real-time immuno-PCR for the quantification of proteins.

Tachi Bar & Ales Tichopad
LabonNet, Israel
Efficiency estimation from single amplification curve - methods and

Bob Rutledge
Natural Resources Canada, Canadian Forest Service, Laurentian Forestry
Centre,Sainte-Foy, Quebec, Canada
Putting the "quantity " into quantitative PCR:A simplified approach to
the establishment and application of quantitative scale.

Bob Rutledge
Amplification efficiency dynamics and its implications: Developing a
kinetic-based approach for quantitative analysis.

Morten T. Andersen
Bio-Molecular Innovation, LGC, Teddington, Middlesex, UK
The Data Comparability Challenge - Standards and Best Practices.

Jim Huggett
Centre for Infectious Diseases & International Health, University
College London, UK
Infectious disease diagnostic research in Africa; the role of real time

Oliver Geulen
Roche Applied Science, Mannheim, Germany
Accurate Gene Expression Analysis with High Flexibility: Concepts and

Cynthia Potter
Eppendorf UK
Validation of fast PCR protocols with the Eppendorf Mastercycler ep

Guido Hennig
Bayer HealthCare AG, Diagnostics Research Germany Leverkusen
Nucleic acid isolation for diagnostic testing using  Bayer´s magnetic

Olfert Landt
TIB MOLBIOL Syntheselabor GmbH Eresburgstraße, Berlin, Germany
Finding the needle in the haystack - LNA bases enhance SNP detection

Andreas Missel
Associate Director R&D, QIAGEN GmbH, Hilden
Going MULTI - how to easily achieve high multiplexing in real-time

Hilary K. Srere
Bio-Rad Laboratories Europe, Munich, Germany
The Ups and Downs of Gene Regulation:  Validating siRNA Gene Expression
Disruption with RT-qPCR.

L. Scott Basehore
Sr. Research Associate, Stratagene Research & Development Department
Nucleic Acid Stabilization in Cultured Cell and Tissue Lysates for QPCR
Gene Expression Analysis.

Kyle Hooper
Promega Corporation, Woods Hollow Road, Madison, WI, USA
Plexor™ Real-Time Quantitative PCR Systems: Multiplexed assays made

Clemence Beslin
Product manager, Eurogentec, Belgium
qPCR pitfalls - primer and probe design / fluorophore quencher

Thomas KaiserCorbett Research, Sydney, AustraliaThe fitness of a
football team:  High Resolution Melts for the determination of

Mark Andersen
Invitrogen, USA
Two-color multiplex assay for the identification of Orthopoxes viruses
with Real-Time LUX PCR.

Simone Guenther
Applied Biosystems, Applera Deutschland GmbH, Darmstadt, Germany
microRNA expression profiles from real-time PCR classify ES and
differentiated cells.

Ben Sowers
Research Associate, Biosearch Technologies
>From Sequences to Synthesis: Optimal Amplification through Careful
Oligonucleotide Selection.

Marc Valer
Agilent Technologies, Waldbronn, Germany
Standardization of RNA Quality Assessment using the RNA Integrity
Number (RIN) and the 2100 bioanalyzer.


worldwide academic and commercial qPCR Events are listed at
Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, LightCycler University, .....etc.
submit your qPCR event here    events at gene-quantification.info

TATAA Biocenter Open Course in qPCR:


TATAA Biocenter have found that the worldwide demand for training in
the field of quantitative real-time PCR (qPCR) is huge. To coordinate
this we aim to arrange practical courses, the 3-day Core Module and the
1-day Biostatistics Module:

The basic Core Module contains three workshop days:
First workshop day will be directed to people planning or considering
using qPCR in their research and also users not yet fully familiar will
quantitative PCR.
Second day targets more advanced users and people concentrating on
different quantification strategies.
The third day focuses on aspects in sample preparation and reverse
The additional 1-day Biostatistics Module explains statistics
applicable to qPCR and teaches how to use statistics to interpret
real-time PCR gene expression data, and classify samples based on
real-time PCR expression profiling.
Courses contain both, theoretical seminars and practical hands-on
training with experienced supervision.
Practical training will be performed on three different real-time PCR
cyclers, using multiple detection chemistries.
The Biostatistics Module is further based on computer-based
demonstrations. Please bring your own Laptop to the course !
qPCR courses are held in regularly in Göteborg, Sweden and in
Freising-Weihenstephan, Germany (near Munich, very close to the Munich
Airport - MUC). Depending on the occasion different prices may apply.
Also different course modules are available on the different occasions.
Further customized workshops and specialized trainings will be held as
well across Europe and world-wide. TATAA Biocenter Germany courses are
held in cooperation with the Institute of Physiology, located at the
Technical University of Munich, in Freising-Weihenstephan.

Workshop details  =>  3-days core module
Download  => short qPCR basic course description at the TATAA Biocenter
Location   => access to TATAA Biocenter Germany in Freising
Download Map  => access to TATAA Biocenter Germany  (map of
Registration  =>  TATAA Biocenter Workshops  (Germany and world-wide)

Course Occasions 2006:
16th - 19th May 2006
27th - 30th June 2006


Please send the qPCR NEWS to further scientists and friends who are
interested in qPCR and in our Academic & Industrial Information
Platform for qPCR.

best regards

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages

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