total DNA from animal skin

Jayakumar, R R.Jayakumar at roswellpark.org
Tue Mar 28 14:50:58 EST 2006


Are you going to isolate total DNA from the animal skin tissue or from
the bacteria that lives on animal skin? It was not very clear.

Jai 

-----Original Message-----
From: methods-bounces at oat.bio.indiana.edu
[mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of GANESH BABU
Sent: Tuesday, March 28, 2006 9:48 AM
To: methods at magpie.bio.indiana.edu
Subject: total DNA from animal skin

Hi all,
  I am Ganesh Babu, PhD student at Alagappa University. I am working on
identification of bacteria associated with goat skin by 16S rRNA gene
sequencing. 
  Could you tell me the best method for extraction of total DNA from
animal skin for direct amplification of 16S rRNA gene for bacterial
diversity studies?
  Thanking you
  Ganesh Babu

				
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