total DNA from animal skin

3pi 3pi at 3p.sol
Wed Mar 29 00:37:09 EST 2006


Jayakumar, R wrote:

> Are you going to isolate total DNA from the animal skin tissue or from
> the bacteria that lives on animal skin? It was not very clear.
> 
> Jai 

It seems he's aiming to bacterial DNA, but maybe he needs a total DNA 
extraction from the skin...


My suggestion for Ganesh is using a Guanindine/Silica DNA extraction as 
described by Boom et al. (1990), refert to:

http://www.uga.edu/srel/DNA_Lab/TSilicaDNA.rtf

I've used it successfully to extract DNA from wild boars museum skins 
(dessiccated, salt-treated ect..)

regards,
P.


More information about the Methods mailing list