Annealing Temperatures for PCR
R.Jayakumar at roswellpark.org
Thu Mar 30 09:34:45 EST 2006
You can calculate it using several online tools. One of my favourite is
You can also search on google with keywords "annealing temperature
calculator tool". The melting temperature is your annealing
But still the actual Annealing temperature has tto be optimized
through gradient PCR using different conc. Off Mg and salts. The Tm
values only helps to give an idea.
Best of luck
From: methods-bounces at oat.bio.indiana.edu
[mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of
retnohh at mail.mitra.net.id
Sent: Wednesday, March 29, 2006 6:31 PM
To: methods at magpie.bio.indiana.edu
Subject: Annealing Temperatures for PCR
From internet I know that youa an expert in Molecular Biology research.
I have a problem with my student PCR.
If you do not mind, please help me to tell me how to calculate the exact
annealing primers for pair of primers:
5'- TTA GGG CAA GAG ATG GTA AGG -3' and
5'- TTA TAA CAA TGA TGG AGG G -3'
Thank you very much for your kindness.
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