PCR cleanup before digest

Jayakumar, R R.Jayakumar at roswellpark.org
Thu Mar 30 16:55:01 EST 2006


That is new info.  I never heard anything like that.  Anyway, I use only
2 ul of PCR product for my digestion and I guess when I make it up to 50
ul Mg is diluted out.  I get perfect digestions.
   Best ofluck
Jai
 

-----Original Message-----
From: methods-bounces at oat.bio.indiana.edu
[mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of newsnet
customer
Sent: Thursday, March 30, 2006 12:48 AM
To: methods at magpie.bio.indiana.edu
Subject: PCR cleanup before digest

Hi,

Been told to do a PCR cleanup before doing a restriction digest because
the salt (MgCl2) in the PCR mix can affect cleavage efficiency. Anyone
like to comment?


ST
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