Tm optimizing for non-gradient thermocycler

[cloningman]

"TRAN HO QUANG" <quangtranho at yahoo.com> ha scritto nel messaggio
news:mailman.419.1143822588.16885.methods at net.bio.net...
> Dear Netter
>
> Can I optimize the Tm temperature with non-gradient
> thermocyler (Applied Biosystem GenAm PCR 9700)?. Any
> suggesstions are appreciated

As far as I know, there's no way to optimize Tm in a PCR reaction with a
non-gradient thermalcycler.
I'm sorry.

-- 
Cloningman

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