Analyzing transformants

newsnet customer customer at newsnet.com
Thu May 4 05:38:49 EST 2006


Hi

I have transformed GFP gene into a vector.
Then transformed the vector into competant E.coli cells.
I have many colonies.

QUESTION:

How to check if colonies have my gene in the CORRECT orientation by PCR and restriction digest? I think the only way is to sequence the region in both directions. 

Any comments appreciated.

Cheers,
ST


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