chromatin IP troubleshooting

Lynn Denekamp trillion.stars at
Thu May 4 12:00:20 EST 2006

The ChIP assay has quite literally sucked the life out of me over the
past year but I thought THIS TIME it would work. No such luck. My IgG
negative control pulls down a product of the same intensity as the
polII positive control antibody. Both antibodies came with the EZ ChIP
kit (Santa Cruz).

The only advice I garnered from their protocol book was to decrease
the number of PCR cycles and decrease the amount of DNA in the
reaction. Any other suggestions?


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