blackhole at abuse.plus.com
Fri May 5 08:41:54 EST 2006
Historians believe that in newspost
<wqG6g.23191$vy1.10010 at news-server.bigpond.net.au> on Fri, 5 May 2006,
newsnet customer <customer at newsnet.com> penned the following literary
>HERE ARE MY THOUGHTS:
>1. chemically competent cells are not suitable for vector >10kb -
Does ccc expression vector transform and at what frequency? I would work
on the assumption that if it is 10E7 per ug that you get back then
assume 10E3-10-E4 maximum per ug of ligated material.
>2. ligase and/or ATP (in lig. buffer) is not functional/depleted -
Simple to test. Cut something with a 4 cutter RE and see if it is
ligatable using a gel to tell.
I love deadlines. I especially like the whooshing noise they make as
they go flying by.
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