Slow DNA samples
pjie2 at cam.ac.uk
Wed May 10 03:41:56 EST 2006
> Hallo everybody,
> Lately our DNA fragments seemed to be shorter than they should. It
> turned out that our DNA samples are running slower that our markers!, from
> the very begging of the run (see photograph at *
> the markers are in the central lane).
> Any idea what the cause may be?
Can hardly make out anything from that picture, it's so blurred. It doesn't
seem to be a picture of your DNA *or* your marker, rather a picture of the
loading dye during running.
So the most obvious answer for that picture is simply that the marker
contains a different loading dye.
If you're certain it contains the same loading dye, then there's something
in your loading buffers which is affecting the electrochemistry of the dye
and/or your samples.
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