An inquiry

Nasser Mahna nasser.mahna at
Wed May 10 04:18:59 EST 2006

Dear All who have enough experience with restriction enzymes,


I am trying long to clone some genes in some vectors, but strangely the
enzymes are not cutting. I have some restriction enzymes from two companies,
but I found they don’t cut. I don’t know when they have been bought for the
lab. I would appreciate if anybody let me know how long is the half life of
these enzymes. In other words, how long does it take for the enzymes stored
at -20°C to lack their efficiency to cut?


I am looking forward to hearing from any cloning specialist asap.




N. Mahna.



Nasser Mahna


Lab for Fruit Breeding and Biotechnology- K.U.Leuven

W. de Croylaan 42, B-3001 Heverlee-Leuven, Belgium

Tel: 16-322413

Mobile: 477-352089

Fax: 16-322966

E-mail:  <mailto:nasser.mahna at>
nasser.mahna at

              <mailto:n_mahna at> n_mahna at


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