BL21DE3pLysS lysis

Joe Doe None at mail.utexas.edu
Wed May 10 11:58:25 EST 2006


In article <1147161593.134484.283780 at j73g2000cwa.googlegroups.com>,
 "mattia" <mattia.pedotti at uninsubria.it> wrote:

> We work with BL21DE3pLysS and recently most of coltures lysis. We have
> just change antibioticos but we have the same resultes.
> Did someone have the same problem? And do you have an idea in order to
> resolve it?
> Thank you
> Mattia


I would suspect phage contamination. If it is phage contamination a 
phage resistant strain is your best option - decontamination is 
practically very difficult. Phage resistant versions of Bl21(DE3) etc. 
are available from several vendors.  

Roland


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