None at mail.utexas.edu
Wed May 10 11:58:25 EST 2006
In article <1147161593.134484.283780 at j73g2000cwa.googlegroups.com>,
"mattia" <mattia.pedotti at uninsubria.it> wrote:
> We work with BL21DE3pLysS and recently most of coltures lysis. We have
> just change antibioticos but we have the same resultes.
> Did someone have the same problem? And do you have an idea in order to
> resolve it?
> Thank you
I would suspect phage contamination. If it is phage contamination a
phage resistant strain is your best option - decontamination is
practically very difficult. Phage resistant versions of Bl21(DE3) etc.
are available from several vendors.
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