double transformation

willy martin.beusch at alexandria.cc
Fri May 12 11:03:46 EST 2006


hi

i'm trying to do a double transformation of 2 plasmids with the same
ori. i just can't bring them into my cells. i varied the dna amount.
when i make the cell compatible with one plasmid and electroporate the
other one inside i get clones, but i think, and everyone in the lab
tells me that the cells will lose one plasmid.

can u help me

tnx,
willy



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