Sequece of pIT ?

Christiaan Karreman Karreman at onkochemie.uni-duesseldorf.de
Mon May 15 01:11:28 EST 2006


Sorry, not the pIT I have; it is a neor retroviral construct.


Christiaan

Wolfgang Schechinger wrote:

> Dear Christiaan,
>
> all information that I currently have is that is has about 2.6kB (IIRC)
> and that it has the usual elements (lac promoter, colE1 ori, apR,
> M13ori) and some more interesting elements, of which I at the moment
> cannot exactly say if the are proprietary elements of pIT2 or already
> modifications: ribosome binding site, something called pelB leader , a
> myc-his tag and a gIII element for propagating the phage. The library
> itself is between pelB and the myc-His tag, followed by amber and the
> gIII.
>
> In our case, it's used for the phage display of single-chain antibodies
> (screening a Tomlinson ScFv library).
>
> As there are some sequencing results which are hard to interprete, we'd
> need (actually my student does :-) some more information about the
> backbone.
>
> that's all I can provide.
>
> Best regards,
>
> Wolfgang



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