Melting point and amplicon size
3pi at 3p.sol
Tue May 16 05:36:49 EST 2006
I'm thinking about discrimination of amplicons of different sizes, say
about 5 bp at least, by analysis of melting curves in a multiplexed
sybr-green real time pcr assay.
I've red about snp detection by melting point analysis of amplicon sizes
up to 160 bp
I'm thinking if there is any theoretical upper limit in amplicon sizes
for the detection of small base number differences, and how to calculate
any hint is welcome,
thnx and regards.
-- Omnia sunt communia
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