Pfu polymerase mixed with Taq polymerase

Julio Cortazar juliocortazar at o2.pl
Wed May 24 06:39:07 EST 2006


Hi there,

Have heared that one may mix Pfu and Taq polymerases (Fermantas). Taq
is copying Pfu verifying. Have any of u tried this combbination? I'm
planning to introduce two nucleotide changes in 1kb DNA fragment. So
far I made set of 6 PCRs (I'm using so called SOE-PCR method with
overlapping extensions) but my RedTaq polymerase made so many mistakes
that I started to sworn it very badly.

Thanks for advise

I come from a country where less that 1% of country earnings is
subscribed for science. We do not have money for reactions using only
Pfu polymerase, so that's why I asking u these things


Jacob



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