Pfu polymerase mixed with Taq polymerase

Nick Theodorakis nick_theodorakis at hotmail.com
Wed May 24 13:32:22 EST 2006


Julio Cortazar wrote:
> Hi there,
>
> Have heared that one may mix Pfu and Taq polymerases (Fermantas). Taq
> is copying Pfu verifying. Have any of u tried this combbination? I'm
> planning to introduce two nucleotide changes in 1kb DNA fragment. So
> far I made set of 6 PCRs (I'm using so called SOE-PCR method with
> overlapping extensions) but my RedTaq polymerase made so many mistakes
> that I started to sworn it very badly.
>

I believe that is similar to the original Long Accurate PCR protocol by
Wayne Barnes. You may find this discussion helpful:

<http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/aug94.html>

Nick

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Nick Theodorakis
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