Why Heat Incativate Serum used as a Blocking Agent?
w.ingram at uq.edu.au
Fri May 26 01:47:22 EST 2006
Why is it that protocols for immuno techniques like immunofluorescence
that use serum as a blocking agent often stipulate that it should be
Historically, serum for use in cell culture was heat inactivated to
remove heat labile complement, to reduce the risk of complement
molecules damaging cells (unnecessary these days for many sera/cell lines).
Since cells in immuno-procedures are generally dead and fixed prior to
blocking, why is serum often heat inactivated for these protocols? Is it
just a historical hang-up that heat inactivated sera is often used for
blocking (since heat inactivated serum was usually handy), or is there a
genuine reason why it should be used in preference to non-heat
inactivated serum for immunfluorescence?
I'm keen to know the answer.
Wendy Ingram, PhD
Senior Research Officer
RCH Cancer Research Laboratory
Phone: +61 7 3636-9211
Fax: +61 7 3365-5455
Email: w.ingram at uq.edu.au
Postal & Delivery Address:
Level 3, Foundation Building
Department of Paediatrics and Child Health
The University of Queensland
Royal Children's Hospital
Herston, QLD 4029, Australia
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