expression construct question

Aawara Chowdhury aawara at FEMA-trailer.org
Sat May 27 01:10:14 EST 2006


In <mailman.1106.1148651654.16885.methods at net.bio.net>,
 Jayakumar, R <R.Jayakumar at roswellpark.org> wrote:

> Thank you for the information.  Would it be possible to get the name of
> that vector from Aiyar's lab?  I would love to use it for my work.  

The name of the vector is AGP109.  The promoter is the CMV promoter.
Both the IRES elements are from EMCV.  There are plenty of sites in
both MCS.  The selection marker is Hyg.

We've made several cell-lines that express both proteins.  I think
they also have a different version of the double-IRES plasmid that
replicates episomally in human cells.

AC
-- 
Email: echo 142322093203359315271794620168064975321554275890186P | dc


More information about the Methods mailing list